• A novel chimeric avidin with increased thermal stability using DNA shuffling 

      Taskinen, Barbara; Airenne, Tomi; Jänis, Janne; Rahikainen, Rolle; Johnson, Mark S; Kulomaa, Markku S; Hytönen, Vesa P (2014)
      Avidins are a family of proteins widely employed in biotechnology. We have previously shown that functional chimeric mutant proteins can be created from avidin and avidin-related protein 2 using a methodology combining ...
    • Bifunctional Avidin with Covalently Modifiable Ligand Binding Site 

      Leppiniemi, Jenni; Määttä, Juha A E; Hammaren, Henrik; Soikkeli, Mikko; Laitaoja, Mikko; Jänis, Janne; Kulomaa, Markku S; Hytönen, Vesa P (2011)
      The extensive use of avidin and streptavidin in life sciences originates from the extraordinary tight biotin-binding affinity of these tetrameric proteins. Numerous studies have been performed to modify the biotin-binding ...
    • Chicken genome analysis reveals novel genes encoding biotin-binding proteins related to avidin family 

      Niskanen, Einari A; Hytönen, Vesa P; Grapputo, Alessandro; Nordlund, Henri R; Kulomaa, Markku S; Laitinen, Olli H (2005)
      Background A chicken egg contains several biotin-binding proteins (BBPs), whose complete DNA and amino acid sequences are not known. In order to identify and characterise these genes and proteins we studied chicken cDNAs ...
    • Chimeric Avidin – NMR Structure and Dynamics of a 56 kDa Homotetrameric Thermostable Protein 

      Tossavainen, Helena; Kukkurainen, Sampo; Määttä, Juha; Kähkönen, Niklas; Pihlajamaa, Tero; Hytönen, Vesa P; Kulomaa, Markku S; Permi, Perttu (2014)
      Chimeric avidin (ChiAVD) is a product of rational protein engineering remarkably resistant to heat and harsh conditions. In quest of the fundamentals behind factors affecting stability we have elucidated the solution NMR ...
    • Construction of Chimeric Dual-Chain Avidin by Tandem Fusion of the Related Avidins 

      Riihimäki, Tiina A; Kukkurainen, Sampo; Varjonen, Suvi; Hörhä, Jarno; Nyholm, Thomas KM; Kulomaa, Markku S; Hytönen, Vesa P (2011)
      Background Avidin is a chicken egg-white protein with high affinity to vitamin H, also known as D-biotin. Many applications in life science research are based on this strong interaction. Avidin is a homotetrameric protein, ...
    • Internalization of novel non-viral vector TAT-streptavidin into human cells 

      Rinne, Johanna; Albarran, Brian; Jylhävä, Juulia; Ihalainen, Teemu O; Kankaanpää, Pasi; Hytönen, Vesa P; Stayton, Patrick S; Kulomaa, Markku S; Vihinen-Ranta, Maija (2007)
      Background The cell-penetrating peptide derived from the Human immunodeficiency virus-1 transactivator protein Tat possesses the capacity to promote the effective uptake of various cargo molecules across the plasma membrane ...
    • Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein 

      Riihimäki, Tiina A; Hiltunen, Soili; Rangl, Martina; Nordlund, Henri R; Määttä, Juha A E; Ebner, Andreas; Hinterdorfer, Peter; Kulomaa, Markku S; Takkinen, Kristiina; Hytönen, Vesa P (2011)
      Background Engineered proteins, with non-immunoglobulin scaffolds, have become an important alternative to antibodies in many biotechnical and therapeutic applications. When compared to antibodies, tailored proteins may ...
    • Structural and functional characteristics of xenavidin, the first frog avidin from Xenopus tropicalis 

      Määttä, Juha A E; Helppolainen, Satu H; Hytönen, Vesa P; Johnson, Mark S; Kulomaa, Markku S; Airenne, Tomi T; Nordlund, Henri R (2009)
      Background Avidins are proteins with extraordinarily high ligand-binding affinity, a property which is used in a wide array of life science applications. Even though useful for biotechnology and nanotechnology, the ...
    • Structure of bradavidin - C-terminal residues act as intrinsic ligands 

      Leppiniemi, Jenni; Grönroos, Toni; Määttä, Juha A E; Johnson, Mark S; Kulomaa, Markku S; Hytönen, Vesa P; Airenne, Tomi T (2012)
      Abstract Top Bradavidin is a homotetrameric biotin-binding protein from Bradyrhizobium japonicum, a nitrogen fixing and root nodule-forming symbiotic bacterium of the soybean. Wild-type (wt) bradavidin has 138 amino ...
    • Zebavidin - An avidin-like protein from zebrafish 

      Taskinen, Barbara; Zmurko, Joanna; Ojanen, Markus; Kukkurainen, Sampo; Parthiban, Marimuthu; Määttä, Juha; Leppiniemi, Jenni; Jänis, Janne; Parikka, Mataleena; Turpeinen, Hannu; Rämet, Mika; Pesu, Marko; Johnson, Mark; Kulomaa, Markku S; Airenne, Tomi; Hytönen, Vesa P (2013)
      The avidin protein family members are well known for their high affinity towards D-biotin and high structural stability. These properties make avidins valuable tools for a wide range of biotechnology applications. We have ...