Exercise-induced expression of angiogenic growth factors in skeletal muscle and in capillaries of healthy and diabetic mice

TamPub

Näytä suppeat kuvailutiedot

dc.contributor.author Kivelä, Riikka -
dc.contributor.author Silvennoinen, Mika -
dc.contributor.author Lehti, Maarit -
dc.contributor.author Jalava, Sanni -
dc.contributor.author Vihko, Veikko -
dc.contributor.author Kainulainen, Heikki -
dc.date.accessioned 2012-06-17T20:13:51Z
dc.date.available 2012-06-15 06:33:25 -
dc.date.available 2012-06-17T20:13:51Z
dc.date.issued 2008 -
dc.identifier.issn 1475-2840 -
dc.identifier.uri http://tampub.uta.fi/handle/10024/65810
dc.description BioMed Central Open access -
dc.description.abstract Background Diabetes has negative, and exercise training positive, effects on the skeletal muscle vasculature, but the mechanisms are not yet fully understood. In the present experiment the effects of running exercise on the mRNA expression of pro- and antiangiogenic factors were studied in healthy and diabetic skeletal muscle. The responses in capillaries and muscle fibers, collected from the muscle with laser capture microdissection, were also studied separately. Methods Healthy and streptozotocin-induced diabetic mice were divided into sedentary and exercise groups. Exercise was a single bout of 1 h running on a treadmill. Gastrocnemius muscles were harvested 3 h and 6 h post exercise, and angiogenesis-related gene expressions were analyzed with real-time PCR. In addition to muscle homogenates, capillaries and muscle fibers were collected from the muscle with laser capture microdissection method and analyzed for vascular endothelial growth factor-A (VEGF-A) and thrombospondin-1 (TSP-1) mRNA expression. Results Of the proangiogenic factors, VEGF-A and VEGF receptor-2 (VEGFR-2) mRNA expression increased significantly (P < 0.05) in healthy skeletal muscle 6 h post exercise. VEGF-B also showed a similar trend (P = 0.08). No significant change was observed post exercise in diabetic muscles in the expression of VEGF-A, VEGFR-2 or VEGF-B. The expression of angiogenesis inhibitor TSP-1 and angiogenic extracellular matrix protein Cyr61 were significantly increased in diabetic muscles (P < 0.05–0.01). Capillary mRNA expression resembled that in the muscle homogenates, however, the responses were greater in capillaries compared to muscle homogenates and pure muscle fibers. Conclusion The present study is the first to report the effects of a single bout of exercise on the expression of pro- and antiangiogenic factors in diabetic skeletal muscle, and it provides novel data about the separate responses in capillaries and muscle fibers to exercise and diabetes. Diabetic mice seem to have lower angiogenic responses to exercise compared to healthy mice, and they show markedly increased expression of angiogenesis inhibitor TSP-1. Furthermore, exercise-induced VEGF-A expression was shown to be greater in capillaries than in muscle fibers. -
dc.language.iso en -
dc.title Exercise-induced expression of angiogenic growth factors in skeletal muscle and in capillaries of healthy and diabetic mice -
dc.type fi=Artikkeli aikakauslehdessä | en=Journal article| -
dc.identifier.urn urn:nbn:uta-3-575 -
dc.identifier.doi 10.1186/1475-2840-7-13 -
dc.type.version fi=Kustantajan versio | en=Publisher's version| -
dc.subject.okm fi=Biolääketieteet | en=Biomedicine| -
dc.journal.title Cardiovascular Diabetology -
dc.journal.volume 7 -
dc.journal.number 13 -
dc.journal.volumepagerange 1-10 -
dc.oldstats 53 -

Viite kuuluu kokoelmiin:

Näytä suppeat kuvailutiedot