Monitoring and analysis of dynamic growth of human embryonic stem cells: comparison of automated instrumentation and conventional culturing methods

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dc.contributor.author Narkilahti, Susanna -
dc.contributor.author Rajala, Kristiina -
dc.contributor.author Pihlajamäki, Harri -
dc.contributor.author Suuronen, Riitta -
dc.contributor.author Hovatta, Outi -
dc.contributor.author Skottman, Heli -
dc.date.accessioned 2012-06-17T20:14:02Z
dc.date.available 2012-06-12 08:51:27 -
dc.date.available 2012-06-17T20:14:02Z
dc.date.issued 2007 -
dc.identifier.issn 1475-925X -
dc.identifier.uri http://tampub.uta.fi/handle/10024/65843
dc.description BioMed Central Open access -
dc.description.abstract Background Human embryonic stem cells (hESCs) are a potential source of cells for use in regenerative medicine. Automation of culturing, monitoring and analysis is crucial for fast and reliable optimization of hESC culturing methods. Continuous monitoring of living cell cultures can reveal more information and is faster than using laborious traditional methods such as microscopic evaluation, immunohistochemistry and flow cytometry. Methods We analyzed the growth dynamics of two hESC lines HS237 and HS293 in a conventional culture medium containing serum replacement and a xeno-free X-vivo 10 medium. We used a new automated culture platform utilizing machine vision technology, which enables automatic observation, recording and analysis of intact living cells. We validated the results using flow cytometry for cell counting and characterization. Results In our analyses, hESC colony growth could be continuously monitored and the proportion of undifferentiated cells automatically analyzed. No labeling was needed and we could, for the first time, perform detailed follow up of live, undisturbed cell colonies, and record all the events in the culture. The growth rate of the hESCs cultured in X-vivo 10 medium was significantly lower and a larger proportion of the cells were differentiated. Conclusion The new automated system enables rapid and reliable analysis of undifferentiated growth dynamics of hESCs. We demonstrate the effectiveness of the system by comparing hESC growth in different culture conditions. -
dc.language.iso en -
dc.title Monitoring and analysis of dynamic growth of human embryonic stem cells: comparison of automated instrumentation and conventional culturing methods -
dc.type fi=Artikkeli aikakauslehdessä | en=Journal article| -
dc.identifier.urn urn:nbn:uta-3-606 -
dc.identifier.doi 10.1186/1475-925X-6-11 -
dc.type.version fi=Kustantajan versio | en=Publisher's version| -
dc.subject.okm fi=Lääketieteen bioteknologia | en=Medical biotechnology| -
dc.journal.title BioMedical Engineering OnLine -
dc.journal.volume 6 -
dc.journal.number 11 -
dc.journal.volumepagerange 1-8 -
dc.oldstats 61 -

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