Inhibition of p38 mitogen-activated protein kinase enhances c-Jun N-terminal kinase activity: Implication in inducible nitric oxide synthase expression

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dc.contributor.author Lahti, Aleksi -
dc.contributor.author Sareila, Outi -
dc.contributor.author Kankaanranta, Hannu -
dc.contributor.author Moilanen, Eeva -
dc.date.accessioned 2012-06-17T20:14:12Z
dc.date.available 2012-06-15 03:30:22 -
dc.date.available 2012-06-17T20:14:12Z
dc.date.issued 2006 -
dc.identifier.issn 1471-2210 -
dc.identifier.uri http://tampub.uta.fi/handle/10024/65874
dc.description BioMed Central Open access -
dc.description.abstract Background Nitric oxide (NO) is an inflammatory mediator, which acts as a cytotoxic agent and modulates immune responses and inflammation. p38 mitogen-activated protein kinase (MAPK) signal transduction pathway is activated by chemical and physical stress and regulates immune responses. Previous studies have shown that p38 MAPK pathway regulates NO production induced by inflammatory stimuli. The aim of the present study was to investigate the mechanisms involved in the regulation of inducible NO synthesis by p38 MAPK pathway. Results p38 MAPK inhibitors SB203580 and SB220025 stimulated lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS) expression and NO production in J774.2 murine macrophages. Increased iNOS mRNA expression was associated with reduced degradation of iNOS mRNA. Treatment with SB220025 increased also LPS-induced c-Jun N-terminal kinase (JNK) activity. Interestingly, JNK inhibitor SP600125 reversed the effect of SB220025 on LPS-induced iNOS mRNA expression and NO production. Conclusion The results suggest that inhibition of p38 MAPK by SB220025 results in increased JNK activity, which leads to stabilisation of iNOS mRNA, to enhanced iNOS expression and to increased NO production. -
dc.language.iso en -
dc.title Inhibition of p38 mitogen-activated protein kinase enhances c-Jun N-terminal kinase activity: Implication in inducible nitric oxide synthase expression -
dc.type fi=Artikkeli aikakauslehdessä | en=Journal article| -
dc.identifier.urn urn:nbn:uta-3-636 -
dc.identifier.doi 10.1186/1471-2210-6-5 -
dc.type.version fi=Kustantajan versio | en=Publisher's version| -
dc.subject.okm fi=Farmasia | en=Pharmacy| -
dc.journal.title BMC Pharmacology -
dc.journal.volume 6 -
dc.journal.number 5 -
dc.journal.volumepagerange 1-12 -
dc.oldstats 60 -

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