Parvovirus Induced Alterations in Nuclear Architecture and Dynamics


Näytä suppeat kuvailutiedot Ihalainen, Teemu O - Niskanen, Einari A - Jylhävä, Juulia - Paloheimo, Outi - Dross, Nicolas - Smolander, Hanna - Langowski, Jörg - Timonen, Jussi - Vihinen-Ranta, Maija - 2012-06-17T20:14:30Z 2012-06-13 10:54:53 - 2012-06-17T20:14:30Z 2009 -
dc.identifier.issn 1932-6203 -
dc.description Public Library of Science -
dc.description.abstract The nucleus of interphase eukaryotic cell is a highly compartmentalized structure containing the three-dimensional network of chromatin and numerous proteinaceous subcompartments. DNA viruses induce profound changes in the intranuclear structures of their host cells. We are applying a combination of confocal imaging including photobleaching microscopy and computational methods to analyze the modifications of nuclear architecture and dynamics in parvovirus infected cells. Upon canine parvovirus infection, expansion of the viral replication compartment is accompanied by chromatin marginalization to the vicinity of the nuclear membrane. Dextran microinjection and fluorescence recovery after photobleaching (FRAP) studies revealed the homogeneity of this compartment. Markedly, in spite of increase in viral DNA content of the nucleus, a significant increase in the protein mobility was observed in infected compared to non-infected cells. Moreover, analyzis of the dynamics of photoactivable capsid protein demonstrated rapid intranuclear dynamics of viral capsids. Finally, quantitative FRAP and cellular modelling were used to determine the duration of viral genome replication. Altogether, our findings indicate that parvoviruses modify the nuclear structure and dynamics extensively. Intranuclear crowding of viral components leads to enlargement of the interchromosomal domain and to chromatin marginalization via depletion attraction. In conclusion, parvoviruses provide a useful model system for understanding the mechanisms of virus-induced intranuclear modifications. -
dc.language.iso en -
dc.title Parvovirus Induced Alterations in Nuclear Architecture and Dynamics -
dc.type fi=Artikkeli aikakauslehdessä | en=Journal article| -
dc.identifier.urn urn:nbn:uta-3-685 -
dc.identifier.doi 10.1371/journal.pone.0005948 -
dc.type.version fi=Kustantajan versio | en=Publisher's version| -
dc.subject.okm fi=Lääketieteen bioteknologia | en=Medical biotechnology| -
dc.journal.title PLoS ONE -
dc.journal.volume 4 -
dc.journal.number 6 -
dc.journal.volumepagerange 1-16 -
dc.oldstats 48 -

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