The Most Recently Discovered Carbonic Anhydrase, CA XV, Is Expressed in the Thick Ascending Limb of Henle and in the Collecting Ducts of Mouse Kidney

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dc.contributor.author Saari, Sina -
dc.contributor.author Hilvo, Mika -
dc.contributor.author Pan, Pei-Wen -
dc.contributor.author Gros, Gerolf -
dc.contributor.author Hanke, Nina -
dc.contributor.author Waheed, Abdul -
dc.contributor.author Sly, William S -
dc.contributor.author Parkkila, Seppo -
dc.date.accessioned 2012-06-17T20:14:32Z
dc.date.available 2012-06-08 03:27:17 -
dc.date.available 2012-06-17T20:14:32Z
dc.date.issued 2010 -
dc.identifier.issn 1932-6203 -
dc.identifier.uri http://tampub.uta.fi/handle/10024/65936
dc.description Public Library of Science -
dc.description.abstract Background Carbonic anhydrases (CAs) are key enzymes for physiological pH regulation, including the process of urine acidification. Previous studies have identified seven cytosolic or membrane-bound CA isozymes in the kidney. Recently, we showed by in situ hybridization that the mRNA for the most novel CA isozyme, CA XV, is present in the renal cortex. CA XV is a unique isozyme among mammalian CAs, because it has become a pseudogene in primates even though expressed in several other species. Methodology/Principal Findings In the present study, we raised a polyclonal antibody against recombinant mouse CA XV that was produced in a baculovirus/insect cell expression system, and the antibody was used for immunohistochemical analysis in different mouse tissues. Positive immunoreactions were found only in the kidney, where the enzyme showed a very limited distribution pattern. Parallel immunostaining experiments with several other anti-CA sera indicated that CA XV is mainly expressed in the thick ascending limb of Henle and collecting ducts, and the reactions were most prominent in the cortex and outer medulla. Conclusion/Significance Although other studies have proposed a role for CA XV in cell proliferation, its tightly limited distribution may point to a specialized function in the regulation of acid-base homeostasis. -
dc.language.iso en -
dc.title The Most Recently Discovered Carbonic Anhydrase, CA XV, Is Expressed in the Thick Ascending Limb of Henle and in the Collecting Ducts of Mouse Kidney -
dc.type fi=Artikkeli aikakauslehdessä | en=Journal article| -
dc.identifier.urn urn:nbn:uta-3-692 -
dc.identifier.doi 10.1371/journal.pone.0009624 -
dc.type.version fi=Kustantajan versio | en=Publisher's version| -
dc.subject.okm fi=Biolääketieteet | en=Biomedicine| -
dc.journal.title PLoS ONE -
dc.journal.volume 5 -
dc.journal.number 3 -
dc.journal.volumepagerange 1-9 -
dc.oldstats 52 -

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