Dual specificity phosphatase 1 regulates human inducible nitric oxide synthase expression by p38 MAP kinase

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dc.contributor.author Turpeinen, Tuija -
dc.contributor.author Nieminen, Riina -
dc.contributor.author Taimi, Ville -
dc.contributor.author Heittola, Taina -
dc.contributor.author Sareila, Outi -
dc.contributor.author Clark, Andrew R -
dc.contributor.author Moilanen, Eeva -
dc.contributor.author Korhonen, Riku -
dc.date.accessioned 2012-06-17T20:14:38Z
dc.date.available 2012-06-15 06:37:56 -
dc.date.available 2012-06-17T20:14:38Z
dc.date.issued 2011 -
dc.identifier.issn 0962-9351 -
dc.identifier.uri http://tampub.uta.fi/handle/10024/65959
dc.description Hindawi open access -
dc.description.abstract The role of dual specificity phosphatase 1 (DUSP1) in inducible nitric oxide synthase (iNOS) expression in A549 human pulmonary epithelial cells, J774 mouse macrophages and primary mouse bone marrow-derived macrophages (BMMs) was investigated. iNOS expression was induced by a cytokine mixture (TNF, IFNγ and IL-1β) in A549 cells and by LPS in J774 cells, and it was inhibited by p38 MAPK inhibitors SB202190 and BIRB 796. Stimulation with cytokine mixture or LPS enhanced also DUSP1 expression. Down-regulation of DUSP1 by siRNA increased p38 MAPK phosphorylation and iNOS expression in A549 and J774 cells. In addition, LPS-induced iNOS expression was enhanced in BMMs from DUSP1(−/−) mice as compared to that in BMMs from wild-type mice. The results indicate that DUSP1 suppresses iNOS expression by limiting p38 MAPK activity in human and mouse cells. Compounds that enhance DUSP1 expression or modulate its function may be beneficial in diseases complicated with increased iNOS-mediated NO production. -
dc.language.iso en -
dc.title Dual specificity phosphatase 1 regulates human inducible nitric oxide synthase expression by p38 MAP kinase -
dc.type fi=Artikkeli aikakauslehdessä | en=Journal article| -
dc.identifier.urn urn:nbn:uta-3-714 -
dc.identifier.doi 10.1155/2011/127587 -
dc.type.version fi=Kustantajan versio | en=Publisher's version| -
dc.subject.okm fi=Biolääketieteet | en=Biomedicine| -
dc.administrativeunit fi=Lääketieteen yksikkö | en=School of Medicine| -
dc.journal.title Mediators of Inflammation -
dc.journal.volume 2011 -
dc.journal.number 127587 -
dc.journal.volumepagerange 1-15 -
dc.oldstats 57 -

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