Leptin enhances synthesis of proinflammatory mediators in human osteoarthritic cartilage mediator role of NO in leptin-induced PGE2, IL-6, and IL-8 production

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dc.contributor.author Vuolteenaho, Katriina -
dc.contributor.author Koskinen, Anna -
dc.contributor.author Kukkonen, Meiju -
dc.contributor.author Nieminen, Riina -
dc.contributor.author Päivärinta, Unto -
dc.contributor.author Moilanen, Teemu -
dc.contributor.author Moilanen, Eeva -
dc.date.accessioned 2012-06-17T20:14:39Z
dc.date.available 2012-06-15 03:41:09 -
dc.date.available 2012-06-17T20:14:39Z
dc.date.issued 2009 -
dc.identifier.issn 1466-1861 -
dc.identifier.uri http://tampub.uta.fi/handle/10024/65966
dc.description Hindawi Open access -
dc.description.abstract Obesity is an important risk factor for osteoarthritis (OA) in weight-bearing joints, but also in hand joints, pointing to an obesityrelated metabolic factor that influences on the pathogenesis of OA. Leptin is an adipokine regulating energy balance, and it has recently been related also to arthritis and inflammation as a proinflammatory factor. In the present paper, the effects of leptin on human OA cartilage were studied. Leptin alone or in combination with IL-1 enhanced the expression of iNOS and COX-2, and production of NO, PGE2, IL-6, and IL-8. The results suggest that the effects of leptin are mediated through activation of transcription factor nuclear factor κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathway c-Jun NH2-terminal kinase (JNK). Interestingly, inhibition of leptin-induced NO production with a selective iNOS inhibitor 1400 W inhibited also the production of IL-6, IL-8, and PGE2, and this was reversed by exogenously added NO-donor SNAP, suggesting that the effects of leptin on IL-6, IL-8, and PGE2 production are dependent on NO. These findings support the idea of leptin as a factor enhancing the production of proinflammatory factors in OA cartilage and as an agent contributing to the obesity-associated increased risk for osteoarthritis. -
dc.language.iso en -
dc.title Leptin enhances synthesis of proinflammatory mediators in human osteoarthritic cartilage mediator role of NO in leptin-induced PGE2, IL-6, and IL-8 production -
dc.type fi=Artikkeli aikakauslehdessä | en=Journal article| -
dc.identifier.urn urn:nbn:uta-3-722 -
dc.identifier.doi 10.1155/2009/345838 -
dc.type.version fi=Kustantajan versio | en=Publisher's version| -
dc.subject.okm fi=Farmasia | en=Pharmacy| -
dc.journal.title Mediators of Inflammation -
dc.journal.volume 2009 -
dc.journal.number 345838 -
dc.journal.volumepagerange 1-10 -
dc.oldstats 58 -

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