Ringer's lactate improves liver recovery in a murine model of acetaminophen toxicity

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dc.contributor.author Yang, Runkuan -
dc.contributor.author Zhang, Shutian -
dc.contributor.author Kajander, Henri -
dc.contributor.author Zhu, Shengtao -
dc.contributor.author Koskinen, Marja-Leena -
dc.contributor.author Tenhunen, Jyrki -
dc.date.accessioned 2012-06-17T20:14:47Z
dc.date.available 2012-06-17 16:39:50 -
dc.date.available 2012-06-17T20:14:47Z
dc.date.issued 2011 -
dc.identifier.issn 1471-230X -
dc.identifier.uri http://tampub.uta.fi/handle/10024/65996
dc.description BioMed Central Open access -
dc.description.abstract Background Acetaminophen (APAP) overdose induces massive hepatocyte necrosis. Liver regeneration is a vital process for survival after a toxic insult. Since hepatocytes are mostly in a quiescent state (G0), the regeneration process requires the priming of hepatocytes by cytokines such as TNF-α and IL-6. Ringer's lactate solution (RLS) has been shown to increase serum TNF-α and IL-6 in patients and experimental animals; in addition, RLS also provides lactate, which can be used as an alternative metabolic fuel to meet the higher energy demand by liver regeneration. Therefore, we tested whether RLS therapy improves liver recovery after APAP overdose. Methods C57BL/6 male mice were intraperitoneally injected with a single dose of APAP (300 mg/kg dissolved in 1 mL sterile saline). Following 2 hrs of APAP challenge, the mice were given 1 mL RLS or Saline treatment every 12 hours for a total of 72 hours. Results 72 hrs after APAP challenge, compared to saline-treated group, RLS treatment significantly lowered serum transaminases (ALT/AST) and improved liver recovery seen in histopathology. This beneficial effect was associated with increased hepatic tissue TNF-α concentration, enhanced hepatic NF-κB DNA binding and increased expression of cell cycle protein cyclin D1, three important factors in liver regeneration. Conclusion RLS improves liver recovery from APAP hepatotoxicity. -
dc.language.iso en -
dc.title Ringer's lactate improves liver recovery in a murine model of acetaminophen toxicity -
dc.type fi=Artikkeli aikakauslehdessä | en=Journal article| -
dc.identifier.urn urn:nbn:uta-3-751 -
dc.identifier.doi 10.1186/1471-230X-11-125 -
dc.type.version fi=Kustantajan versio | en=Publisher's version| -
dc.subject.okm fi=Sisätaudit | en=Internal Medicine| -
dc.administrativeunit fi=Lääketieteen yksikkö | en=School of Medicine| -
dc.journal.title BMC Gastroenterology -
dc.journal.volume 11 -
dc.journal.number 125 -
dc.journal.volumepagerange 1-10 -
dc.oldstats 60 -

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