Adiponectin associates with markers of cartilage degradation in osteoarthritis and induces production of proinflammatory and catabolic factors through mitogen activated protein kinase pathways

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dc.contributor.author Koskinen, Anna -
dc.contributor.author Juslin, Sami -
dc.contributor.author Nieminen, Riina -
dc.contributor.author Moilanen, Teemu -
dc.contributor.author Vuolteenaho, Katriina -
dc.contributor.author Moilanen, Eeva -
dc.date.accessioned 2012-06-17T20:14:53Z
dc.date.available 2012-06-17 16:09:26 -
dc.date.available 2012-06-17T20:14:53Z
dc.date.issued 2011 -
dc.identifier.issn 1478-6354 -
dc.identifier.uri http://tampub.uta.fi/handle/10024/66014
dc.description BioMed Central open access -
dc.description.abstract Introduction Adiponectin is an adipokine that regulates energy metabolism and insulin sensitivity, but recent studies have pointed also to a role in inflammation and arthritis. The purpose of the present study was to investigate the association and effects of adiponectin on inflammation and cartilage destruction in osteoarthritis (OA). Methods Cartilage and blood samples were collected from 35 male OA patients undergoing total knee replacement surgery. Preoperative radiographs were evaluated by Ahlback classification criteria for knee OA. Circulating concentrations of adiponectin and biomarkers of OA, i.e. cartilage oligomeric matrix protein (COMP) and matrix metalloproteinase 3 (MMP-3), were measured. Cartilage samples obtained at the surgery were cultured ex vivo and the levels of adiponectin, nitric oxide (NO), interleukin-6 (IL-6) and metalloproteinases MMP-1 and MMP-3 were determined in the culture media. In addition, the effects of adiponectin on the production of NO, IL-6, MMP-1 and MMP-3 were studied in cartilage and in primary chondrocyte cultures. Results Plasma adiponectin levels and adiponectin released from OA cartilage were higher in patients with radiologically most severe OA (Ahlback grades 4-5) than in patients with less severe disease (grades 1-3). Plasma adiponectin concentrations correlated positively with biomarkers of OA, i.e. COMP (r = 0.55, p = 0.001) and MMP-3 (r = 0.34, p = 0.046). Adiponectin was released by OA cartilage ex vivo and it correlated positively with NO (r = 0.43, p = 0.012), IL-6 (r = 0.42, p = 0.018), and MMP-3 (r = 0.34, p = 0.051) production. Further, adiponectin enhanced production of NO, IL-6, MMP-1 and MMP-3 in OA cartilage and in primary chondrocytes in vitro by a mitogen-activated protein kinase (MAPK) dependent manner. Conclusions These findings show that adiponectin is associated with and possibly mediates cartilage destruction in OA. -
dc.language.iso en -
dc.title Adiponectin associates with markers of cartilage degradation in osteoarthritis and induces production of proinflammatory and catabolic factors through mitogen activated protein kinase pathways -
dc.type fi=Artikkeli aikakauslehdessä | en=Journal article| -
dc.identifier.urn urn:nbn:uta-3-766 -
dc.identifier.doi doi:10.1186/ar3512 -
dc.type.version fi=Kustantajan versio | en=Publisher's version| -
dc.subject.okm fi=Biokemia, solu- ja molekyylibiologia | en=Biochemistry, cell and molecular biology| -
dc.administrativeunit fi=Lääketieteen yksikkö | en=School of Medicine| -
dc.journal.title Arthritis Research & Therapy -
dc.journal.volume 13 -
dc.journal.number 6 -
dc.journal.volumepagerange R184 -
dc.oldstats 65 -

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