Manufacturing and in vivo inner ear visualization of MRI traceable liposome nanoparticles encapsulating gadolinium


Näytä suppeat kuvailutiedot Zou, Jing - Sood, Rohit - Ranjan, Sanjeev - Poe, Dennis - Ramadan, Usama A - Kinnunen, Paavo KJ - Pyykkö, Ilmari - 2012-06-17T20:15:51Z 2012-06-15 06:16:30 - 2012-06-17T20:15:51Z 2010 -
dc.identifier.issn 1477-3155 -
dc.description BioMed Central Open access -
dc.description.abstract Background Treatment of inner ear diseases remains a problem because of limited passage through the blood-inner ear barriers and lack of control with the delivery of treatment agents by intravenous or oral administration. As a minimally-invasive approach, intratympanic delivery of multifunctional nanoparticles (MFNPs) carrying genes or drugs to the inner ear is a future therapy for treating inner ear diseases, including sensorineural hearing loss (SNHL) and Meniere's disease. In an attempt to track the dynamics and distribution of nanoparticles in vivo, here we describe manufacturing MRI traceable liposome nanoparticles by encapsulating gadolinium-tetra-azacyclo-dodecane-tetra-acetic acid (Gd-DOTA) (abbreviated as LPS+Gd-DOTA) and their distribution in the inner ear after either intratympanic or intracochlear administration. Results Measurements of relaxivities (r1 and r2) showed that LPS+Gd-DOTA had efficient visible signal characteristics for MRI. In vivo studies demonstrated that LPS+Gd-DOTA with 130 nm size were efficiently taken up by the inner ear at 3 h after transtympanic injection and disappeared after 24 h. With intracochlear injection, LPS+Gd-DOTA were visualized to distribute throughout the inner ear, including the cochlea and vestibule with fast dynamics depending on the status of the perilymph circulation. Conclusion Novel LPS+Gd-DOTA were visible by MRI in the inner ear in vivo demonstrating transport from the middle ear to the inner ear and with dynamics that correlated to the status of the perilymph circulation. -
dc.language.iso en -
dc.title Manufacturing and in vivo inner ear visualization of MRI traceable liposome nanoparticles encapsulating gadolinium -
dc.type fi=Artikkeli aikakauslehdessä | en=Journal article| -
dc.identifier.urn urn:nbn:uta-3-499 -
dc.identifier.doi 10.1186/1477-3155-8-32 -
dc.type.version fi=Kustantajan versio | en=Publisher's version| -
dc.subject.okm fi=Korva-, nenä- ja kurkkutaudit, silmätaudit | en=Otorhinolaryngology, ophthalmology| -
dc.journal.title Journal of Nanobiotechnology -
dc.journal.volume 8 -
dc.journal.number 32 -
dc.journal.volumepagerange 1-11 -
dc.oldstats 109 -

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