The plasma membrane carbonic anhydrase in murine hepatocytes identified as isozyme XIV


Näytä suppeat kuvailutiedot Parkkila, Seppo - Kivelä, Antti J - Kaunisto, Kari - Parkkila, Anna-Kaisa - Hakkola, Jukka - Rajaniemi, Hannu - Waheed, Abdul - Sly, William S - 2012-06-17T20:16:27Z 2012-06-16 09:01:55 - 2012-06-17T20:16:27Z 2002 -
dc.identifier.issn 1471-230X -
dc.description BioMed Central Open access -
dc.description.abstract Background Biochemical and histochemical studies have both previously indicated plasma membrane-associated carbonic anhydrase (CA) activity in hepatocytes which has been assumed to be CA IV. However, immunohistochemical data did not support this assignment. Recent northern blotting results indicated the presence of mRNA for the most recently discovered membrane-bound CA isozyme, CA XIV, in the liver. The present study was designed to examine whether CA XIV could contribute to the CA activity described in the hepatocytes. Methods Tissue samples from mouse liver were subjected to immunohistochemical staining using the antibodies raised against recombinant mouse CA XIV and CA IV. RT-PCR and western blotting were also performed for CA XIV. Results A strong immunofluorescent signal was observed in the plasma membrane of mouse hepatocytes. Although CA XIV was expressed on both the apical and basolateral surfaces, the staining was more prominent at the apical (canalicular) membrane domain. The expression of CA XIV in the liver was confirmed by RT-PCR and western blotting. Conclusions The presence of CA XIV in the hepatocyte plasma membrane places this novel enzyme at a strategic site to control pH regulation and ion transport between the hepatocytes, sinusoids and bile canaliculi. -
dc.language.iso en -
dc.title The plasma membrane carbonic anhydrase in murine hepatocytes identified as isozyme XIV -
dc.type fi=Artikkeli aikakauslehdessä | en=Journal article| -
dc.identifier.urn urn:nbn:uta-3-516 -
dc.identifier.doi 10.1186/1471-230X-2-13 -
dc.type.version fi=Kustantajan versio | en=Publisher's version| -
dc.subject.okm fi=Biolääketieteet | en=Biomedicine| -
dc.journal.title BMC Gastroenterology -
dc.journal.volume 13 -
dc.journal.number 2 -
dc.journal.volumepagerange 1-7 -
dc.oldstats 88 -

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