Histone deacetylase inhibitors induce apoptosis in human eosinophils and neutrophils

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dc.contributor.author Kankaanranta, Hannu -
dc.contributor.author Janka-Junttila, Mirkka -
dc.contributor.author Ilmarinen-Salo, Pinja -
dc.contributor.author Ito, Kazuhiro -
dc.contributor.author Jalonen, Ulla -
dc.contributor.author Ito, Misako -
dc.contributor.author Adcock, Ian -
dc.contributor.author Moilanen, Eeva -
dc.contributor.author Zhang, Xianzhi -
dc.date.accessioned 2012-06-17T20:16:36Z
dc.date.available 2012-06-15 14:46:41 -
dc.date.available 2012-06-17T20:16:36Z
dc.date.issued 2010 -
dc.identifier.issn 1476-9255 -
dc.identifier.uri http://tampub.uta.fi/handle/10024/66272
dc.description BioMed Central Open access -
dc.description.abstract Background Granulocytes are important in the pathogenesis of several inflammatory diseases. Apoptosis is pivotal in the resolution of inflammation. Apoptosis in malignant cells is induced by histone deacetylase (HDAC) inhibitors, whereas HDAC inhibitors do not usually induce apoptosis in non-malignant cells. The aim of the present study was to explore the effects of HDAC inhibitors on apoptosis in human eosinophils and neutrophils. Methods Apoptosis was assessed by relative DNA fragmentation assay, annexin-V binding, and morphologic analysis. HDAC activity in nuclear extracts was measured with a nonisotopic assay. HDAC expression was measured by real-time PCR. Results A HDAC inhibitor Trichostatin A (TSA) induced apoptosis in the presence of survival-prolonging cytokines interleukin-5 and granulocyte-macrophage colony stimulating factor (GM-CSF) in eosinophils and neutrophils. TSA enhanced constitutive eosinophil and neutrophil apoptosis. Similar effects were seen with a structurally dissimilar HDAC inhibitor apicidin. TSA showed additive effect on the glucocorticoid-induced eosinophil apoptosis, but antagonized glucocorticoid-induced neutrophil survival. Eosinophils and neutrophils expressed all HDACs at the mRNA level except that HDAC5 and HDAC11 mRNA expression was very low in both cell types, HDAC8 mRNA was very low in neutrophils and HDAC9 mRNA low in eosinophils. TSA reduced eosinophil and neutrophil nuclear HDAC activities by ~50-60%, suggesting a non-histone target. However, TSA did not increase the acetylation of a non-histone target NF-κB p65. c-jun-N-terminal kinase and caspases 3 and 6 may be involved in the mechanism of TSA-induced apoptosis, whereas PI3-kinase and caspase 8 are not. Conclusions HDAC inhibitors enhance apoptosis in human eosinophils and neutrophils in the absence and presence of survival-prolonging cytokines and glucocorticoids. -
dc.language.iso en -
dc.title Histone deacetylase inhibitors induce apoptosis in human eosinophils and neutrophils -
dc.type fi=Artikkeli aikakauslehdessä | en=Journal article| -
dc.identifier.urn urn:nbn:uta-3-535 -
dc.identifier.doi 10.1186/1476-9255-7-9 -
dc.type.version fi=Kustantajan versio | en=Publisher's version| -
dc.subject.okm fi=Biolääketieteet | en=Biomedicine| -
dc.journal.title Journal of Inflammation -
dc.journal.volume 7 -
dc.journal.number 9 -
dc.journal.volumepagerange 1-15 -
dc.oldstats 76 -

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