Arachidonic acid increases matrix metalloproteinase 9 secretion and expression in human monocytic MonoMac 6 cells

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dc.contributor.author Solakivi, Tiina -
dc.contributor.author Kunnas, Tarja -
dc.contributor.author Kärkkäinen, Satu -
dc.contributor.author Jaakkola, Olli -
dc.contributor.author Nikkari, Seppo T -
dc.date.accessioned 2012-06-17T20:16:41Z
dc.date.available 2012-06-17 06:27:20 -
dc.date.available 2012-06-17T20:16:41Z
dc.date.issued 2009 -
dc.identifier.issn 1476-511X -
dc.identifier.uri http://tampub.uta.fi/handle/10024/66293
dc.description BioMed Central Open access -
dc.description.abstract Background Dietary fatty acids may modulate inflammation in macrophages of the atherosclerotic plaque, affecting its stability. The n-6 polyunsaturated fatty acid (PUFA) arachidonic acid (AA) generally promotes inflammation, while the PUFAs of the n-3 series eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA) are considered anti-inflammatory. We determined how these PUFAs influence MMP-9 expression and secretion by the human monocytic cell line (MonoMac 6) at baseline and after 24-hour exposure. MMP-9 protein was measured by zymography and relative levels of MMP-9 mRNA were determined using quantitative real time PCR. Results Supplementation with AA (but not the n-3 fatty acids) increased, in a dose-dependent manner, expression of MMP-9 protein. This stimulation was regulated at the mRNA level. MMP-9 secretion started after 1 h of incubation and could not be prevented by simultaneous presence of n-3 series fatty acids. Finally, the secretion could be attenuated by LY 294002, a specific phosphatidylinositol-3-kinase (PI3K) inhibitor and by SH-5, a selective Akt inhibitor, suggesting that activation of PI3K by AA leads to augmented and sustained MMP-9 production. Conclusion This study shows that of the PUFA studied, AA alone influences the expression of MMP-9, which might have implications in MMP-9 induced plaque rupture. -
dc.language.iso en -
dc.title Arachidonic acid increases matrix metalloproteinase 9 secretion and expression in human monocytic MonoMac 6 cells -
dc.type fi=Artikkeli aikakauslehdessä | en=Journal article| -
dc.identifier.urn urn:nbn:uta-3-554 -
dc.identifier.doi 10.1186/1476-511X-8-11 -
dc.type.version fi=Kustantajan versio | en=Publisher's version| -
dc.subject.okm fi=Biolääketieteet | en=Biomedicine| -
dc.journal.title Lipids in Health and Disease -
dc.journal.volume 8 -
dc.journal.number 11 -
dc.journal.volumepagerange 1-8 -
dc.oldstats 62 -

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