Convergent Evolution of Argonaute-2 Slicer Antagonism in Two Distinct Insect RNA Viruses


Näytä suppeat kuvailutiedot van Mierlo, Joel Bronkhorst, Alfred Overheul, Gijs Sadanandan, Sajna Ekström, Jens-Ola Heestermans, Marco Hultmark, Dan Antoniewski, Chistophe van Rij, Ronald 2012-10-16T12:21:13Z 2012-10-16T12:21:13Z 2012
dc.identifier.issn 1553-7366 -
dc.description Public Library of Science open access -
dc.description.abstract RNA interference (RNAi) is a major antiviral pathway that shapes evolution of RNA viruses. We show here that Nora virus, a natural Drosophila pathogen, is both a target and suppressor of RNAi. We detected viral small RNAs with a signature of Dicer-2 dependent small interfering RNAs in Nora virus infected Drosophila. Furthermore, we demonstrate that the Nora virus VP1 protein contains RNAi suppressive activity in vitro and in vivo that enhances pathogenicity of recombinant Sindbis virus in an RNAi dependent manner. Nora virus VP1 and the viral suppressor of RNAi of Cricket paralysis virus (1A) antagonized Argonaute-2 (AGO2) Slicer activity of RNA induced silencing complexes pre-loaded with a methylated single-stranded guide strand. The convergent evolution of AGO2 suppression in two unrelated insect RNA viruses highlights the importance of AGO2 in antiviral defense. -
dc.language.iso en -
dc.title Convergent Evolution of Argonaute-2 Slicer Antagonism in Two Distinct Insect RNA Viruses -
dc.type fi=Artikkeli aikakauslehdessä | en=Journal Article| -
dc.identifier.urn URN:NBN:fi:uta-201210161057 -
dc.identifier.doi 10.1371/journal.ppat.1002872 -
dc.type.version fi=Kustantajan versio | en=Publisher's version| -
dc.subject.okm fi=Lääketieteen bioteknologia | en=Medical biotechnology| -
dc.administrativeunit fi=Biolääketieteellisen teknologian yksikkö | en=Institute of Biomedical Technology| -
dc.journal.title Plos Pathogens -
dc.journal.volume 8 -
dc.journal.number 8 -
dc.journal.volumepagerange 1-13 -
dc.journal.url -
dc.ismoreauthorsthanlisted 0 -

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Näytä suppeat kuvailutiedot