Convergent Evolution of Argonaute-2 Slicer Antagonism in Two Distinct Insect RNA Viruses

TamPub

Näytä suppeat kuvailutiedot

dc.contributor.author van Mierlo, Joel
dc.contributor.author Bronkhorst, Alfred
dc.contributor.author Overheul, Gijs
dc.contributor.author Sadanandan, Sajna
dc.contributor.author Ekström, Jens-Ola
dc.contributor.author Heestermans, Marco
dc.contributor.author Hultmark, Dan
dc.contributor.author Antoniewski, Chistophe
dc.contributor.author van Rij, Ronald
dc.date.accessioned 2012-10-16T12:21:13Z
dc.date.available 2012-10-16T12:21:13Z
dc.date.issued 2012
dc.identifier.issn 1553-7366 -
dc.identifier.uri http://tampub.uta.fi/handle/10024/66359
dc.description Public Library of Science open access -
dc.description.abstract RNA interference (RNAi) is a major antiviral pathway that shapes evolution of RNA viruses. We show here that Nora virus, a natural Drosophila pathogen, is both a target and suppressor of RNAi. We detected viral small RNAs with a signature of Dicer-2 dependent small interfering RNAs in Nora virus infected Drosophila. Furthermore, we demonstrate that the Nora virus VP1 protein contains RNAi suppressive activity in vitro and in vivo that enhances pathogenicity of recombinant Sindbis virus in an RNAi dependent manner. Nora virus VP1 and the viral suppressor of RNAi of Cricket paralysis virus (1A) antagonized Argonaute-2 (AGO2) Slicer activity of RNA induced silencing complexes pre-loaded with a methylated single-stranded guide strand. The convergent evolution of AGO2 suppression in two unrelated insect RNA viruses highlights the importance of AGO2 in antiviral defense. -
dc.language.iso en -
dc.title Convergent Evolution of Argonaute-2 Slicer Antagonism in Two Distinct Insect RNA Viruses -
dc.type fi=Artikkeli aikakauslehdessä | en=Journal Article| -
dc.identifier.urn URN:NBN:fi:uta-201210161057 -
dc.identifier.doi 10.1371/journal.ppat.1002872 -
dc.type.version fi=Kustantajan versio | en=Publisher's version| -
dc.subject.okm fi=Lääketieteen bioteknologia | en=Medical biotechnology| -
dc.administrativeunit fi=Biolääketieteellisen teknologian yksikkö | en=Institute of Biomedical Technology| -
dc.journal.title Plos Pathogens -
dc.journal.volume 8 -
dc.journal.number 8 -
dc.journal.volumepagerange 1-13 -
dc.journal.url http://www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1002872 -
dc.ismoreauthorsthanlisted 0 -

Viite kuuluu kokoelmiin:

Näytä suppeat kuvailutiedot